New methods for genetics studies in plants
PhD defence, Monday 20 February 2012. Dorian Urbański.
2012.02.08
Dorian Urbański
During his PhD studies, Dorian Urbański developed new methods for genetics studies in plants, based on single-gene knockout and gene silencing.
In his research, Dorian Urbański used a self-replicating genomic element called Lotus retrotransposon 1 (LORE1) to produce a large population of plants carrying insertions in individual genes. His research resulted in the development of a high throughput methodology for robust mutagenesis and simultaneous identification of LORE1 gene insertions in the thousands of plants making up the population, as well as contributing seeds to a bar-coded seed bank. This genetic resource opens up new possibilities for the functional analysis of plant genes.
The resource is the result of collaboration between the Centre for Carbohydrate Recognition and Signalling (CARB), Aarhus University, and the National Institute of Agrobiological Studies, Japan, and it provides free access to mutant lines for the global plant research community.
The PhD degree was completed at the Centre for Carbohydrate Recognition and Signalling (CARB), Science and Technology, Aarhus University.
Time: Monday 20 February 2012 at 13.00
Place: Aarhus University Conference Centre (Studenterhusfonden), Meeting Room 2, Fredrik Nielsens Vej 2–4, 8000 Aarhus C
Title of dissertation: Expanding the Lotus japonicus reverse genetics toolbox – development of LORE1 retrotransposon mutagenesis and artificial miRNA-mediated silencing
Contact information: Dorian Urbański, dou@mb.au.dk +45 8715 4938
Members of the assessment committee:
Professor Michael Udvari, The Samuel Roberts Noble Foundation, USA
Dr Thomas Didion, DLF-Trifolium A/S, Denmark
Professor Torben Heick Jensen (chair), Department of Molecular Biology and Genetics, Aarhus University
Main supervisor:
Professor Jens Stougaard, Centre for Carbohydrate Recognition and Signalling (CARB), Aarhus University
Co-supervisor:
Dr Stig Uggerhøj Andersen, Centre for Carbohydrate Recognition and Signalling (CARB), Aarhus University
Language: The dissertation will be defended in English
The defence is public.
The dissertation is available for reading at the Graduate School of Science and Technology / GSST, Ny Munkegade 120, building 1521, room 112, 8000 Aarhus C.
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